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規(guī)格

規(guī)格	8 樣本、4 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Label-based
檢測(cè)方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測(cè)靶標(biāo)	IFN-gamma, IL-1 alpha, IL-1 beta, IL-8, MCP-1, MIG, MIP-1 beta, RANTES, TNF alpha, Angiopoietin-1, bFGF, CD40 Ligand, Decorin, GASP-1, IFN-beta, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-21, IL-36 Ra, IL-4, IP-10, LIF, NCAM-1, VEGF-A, CCL4, CXCL10, CXCL9, IL-1 F1, IL-1 F5, GRO alpha, EGFR, ErbB3, Erythropoietin, FGF-7, Galectin-3, GM-CSF, HGF, HGFR, IGFBP-2, IL-1 Ra, IL-12 p40, IL-6, MMP-8, PDGF-BB, RAGE, SCF, TGF alpha, TNF RI, CXCL8, IL-1 F2, IL-1 F3, CCL2, Fas, IL-5, CCL5, IL-16, Pentraxin-3, TSG-14, ACRP30, Adiponectin, AgRP, Angiopoietin-2, Apo-1, CCL16, CCL18, CCL25, CCL7, CXCL5, ENA-78, FGF-6, Growth Hormone, HCC-4, IGF-1 R, IGFBP-1, IGFBP-3, IL-1 R1, IL-1 R4, IL-11, IL-12 p70, IL-6 R, Insulin, Leptin, Leptin R, Lymphotactin, M-CSF, MARC, MCP-3, MIF, Oncostatin M, Osteoprotegerin, PARC, PDGF-AA, PDGF-AB, SAA, Serum Amyloid A, Somatotropin, ST2, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF RII, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF27, TNFRSF6, XCL1, XEDAR, CD106, CXCL16, GPNMB, Lipocalin-2, NGAL, Osteoactivin, VCAM-1, Angiogenin, Angiostatin, CCL1, CCL13, CD31, CXCL11, EGF, Endostatin, GCSF, GRO alpha/beta/gamma, I-309, I-TAC, KDR, MCP-4, MMP-1, MMP-9, PECAM-1, PLGF, TCA-3, Thrombopoietin, Tie-2, TPO, uPAR, VEGF-D, VEGFR2, VEGFR3, bax, CD40, DR4, DR5, DR6, Fas Ligand, IGF-2, IGFBP-4, IGFBP-6, TNF beta, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF21, TNFRSF5, TNFSF1B, TNFSF5, TNFSF6, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, CCL11, CCL20, CD54, Eotaxin-1, GDNF, IL-1 R2, MIP-3 alpha, TGF beta 2, TGF beta 3, 6Ckine, AcPL, Activin A, Activin B, Activin C, Activin R1A, Activin R1B, Activin R2A, Activin R2A/B, AK155, ALCAM, ALK-1, ALK-2, ALK-3, ALK-4, ALK-5, ALK-6, Alpha Chain and Full Length, Amphiregulin, Angiopoietin-3, Angiopoietin-4, ANGPTL1, ANGPTL2, ANGPTL7, Apelin Receptor, APJ, APRIL, Artemin, AXL, B7-1, BACE-1, BAFF R, BCMA, BD-1, BDNF, beta-Catenin, beta-NGF, Betacellulin, BIK, BLC, BLR-1, BMP-11, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPER, BMPR-IA, BMPR-IB, BMPR-II, BRAK, BTC, Cardiotrophin-1, CCL14, CCL15, CCL17, CCL19, CCL21, CCL22, CCL24, CCL26, CCL27, CCL28, CCL3, CCN2, CCN3, CCN4, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD102, CD105, CD114, CD117, CD11b, CD122, CD127, CD14, CD152, CD163, CD166, CD170, CD220, CD27, CD30, CD30 Ligand, CD50, CD56, CD62L, CD80, CD91 Cluster II, CDH5, Cerberus 1, CF10, Chem R23, Chordin-Like 2, Chordin-Like-1, CHRDL1, CLC, CNTF, CNTF R alpha, Coagulation Factor III, Coagulation Factor X, Common gamma Chain, CRAM-A/B, CRIM1, Cripto-1, Crossveinless-2, CRTH-2, Cryptic, CSFR1, CSK, CT-1, CTACK, CTGF, CTLA-4, CV-2, CX3CL1, CXCL1, CXCL12, CXCL13, CXCL14, CXCL2, CXCL4, CXCL6, CXCL7, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, D6, DAN, DANCE, DcR3, DKK-1, Dkk-3, Dkk-4, DkkL1, DLK-1, DR3, Dtk, E-Selectin, EDA-A2, EDAR, EDG-1, EG-VEGF, EMAP-II, EML2, EN-RAGE, Endocan, Endoglin, Endothelin-1, Eotaxin-2, Eotaxin-3, Epiregulin, ErbB1, ErbB2, ErbB4, FA1, FACX, FADD, FAM3B, FGF R3, FGF R4, FGF R5, FGF-10, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-8, FGF-9, FGF-BP, FGFRL1, FIL1 delta, FIL1 epsilon, FIL1 eta, FIL1 zeta, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Fusin, GASP-2, GCP-2, GCSF R, GDF-1, GDF-10, GDF-11, GDF-15, GDF-3, GDF-5, GDF-8, GDF-9, GDF-9B, GDNF R, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR, GITR Ligand, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF R alpha, gp130, Granzyme A, Grb2, Gremlin-1, Growth Hormone R, HB-EGF, HCC-1, HCR, Hepassocin, HER4, HFREP-1, HRG1-alpha, HRG1-beta 1, HVEM, ICAM-1, ICAM-2, ICAM-3, ICAM-5, IDE, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-gamma R1, IFN-lambda 1, IFN-lambda 2, IGF-2 R, IGFBP-7, IGFBP-rp1, IL-1 F10, IL-1 F6, IL-1 F7, IL-1 F8, IL-1 F9, IL-1 H1, IL-1 HY2, IL-1 R Acp, IL-1 R3, IL-1 R5, IL-1 R6, IL-1 R8, IL-1 R9, IL-1 RAPL2, IL-1 Rrp2, IL-10 R alpha, IL-10 R beta, IL-12 R beta 1, IL-12 R beta 2, IL-13 R alpha 1, IL-13 R alpha 2, IL-13 R1, IL-15 R alpha, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-18 BP alpha, IL-18 R alpha, IL-18 R beta, IL-19, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-22Ra2, IL-23, IL-23 R, IL-24, IL-25, IL-26, IL-27, IL-27 R alpha, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-37, IL-38, IL-4 R, IL-5 R alpha, IL-7, IL-7 R alpha, IL-8 RA, IL-8 RB, IL-9, IL1RAPL1, INHBC, Insulin R, Insulysin, Integrin alpha-M, ITGAL, KGF, KGF-2, Kininogen, Kininostatin, KITLG, Kremen-1, Kremen-2, L-Selectin, LAP2, LBP, LCK, LECT2, Lefty-A, LFA-1 alpha, LIF R alpha, LIGHT, Lipocalin-1, LRP-1 Cluster II, LRP-6, LTBP1, Lymphotoxin beta, Lymphotoxin beta R, M-CSF R, MAC-1, MADH9, MDC, MEC, MET, MFG-E8, MFRP, MICA, MIP-1 alpha, MIP-1 delta, MIP-2, MIP-3 beta, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MPIF-2, MSP alpha, MT3-MMP, MT5-MMP, MT6-MMP, MuSK, Myostatin, NAP-2, Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGFR, Nidogen-1, NOV, Nr-CAM, NRG1 Isoform GGF2, NRG1-alpha, NRG1-beta 1, NRG2, NRG3, NT-3, NT-4, Orexin-A, Orexin-B, Osteocrin, Osteonectin, OX40 Ligand, P-Selectin, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-C, PDGF-D, Peroxiredoxin-6, Persephin, PK1, Platelet Factor 4, PLUNC, Prdx6, Pref-1, Progranulin, Prolactin, RANK, RELM beta, RELT, ROBO4, S100 A10, S100 A12, S100 A8/A9, SCF R, SDF-1, sFRP-1, sFRP-3, sFRP-4, sgp130, Shh-N, SIGIRR, Siglec-5, Siglec-9, SLPI, SMAD1, SMAD4, SMAD5, SMAD7, SMAD8, SMAD9, Soggy-1, Sonic Hedgehog N-Terminal, SPARC, Spinesin, TACI, TARC, TCCR, TCK-1, TFPI, TGF beta R2, TGF beta R3, TGF-beta 5, TGF-beta RI, Thromboplastin, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymidine Phosphorylase, Thymopoietin isform alpha, Thyroid Peroxidase, Tie-1, TIMP-3, TIMP-4, Tissue Factor, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF1, TMEFF2, TMPRSS5, TNF RIII, TNFRSF11A, TNFRSF12A, TNFRSF13B, TNFRSF13C, TNFRSF14, TNFRSF16, TNFRSF17, TNFRSF18, TNFRSF19, TNFRSF19L, TNFRSF25, TNFRSF3, TNFRSF6B, TNFRSF7, TNFRSF8, TNFSF10, TNFSF11, TNFSF12, TNFSF14, TNFSF15, TNFSF18, TNFSF3, TNFSF4, TNFSF8, Tomoregulin-1, TP, TPX, TRADD, TRAIL, TRANCE, TREM-1, TROY, TSG-6, TSLP R, TWEAK, TWEAK R, TYMP, TYRO3, Ubiquitin+1, uPA, Vasorin, VE-Cadherin, VEGF-B, VEGF-C, WFIKKN, WFIKKNRP, WIF-1, WISP-1, WSX-1
靶標(biāo)數(shù)	507

詳細(xì)描述

Introduction

Protein S-acylation (or protein S-palmitoylation) plays an unusually prominent role in cell signaling, development and growth. By monitoring the changes in protein S-Acylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.

The array kit uses a modified 'biotin-switch' (acyl-biotinyl exchange (ABE)) method (Jaffrey et al.) to allow for the direct visualization of S-acylated proteins on the antibody array. Prior to running the array, the samples are labeled. Unmodified free cysteines on proteins in the sample are first blocked. S-acylated cysteines are then selectively reduced for specific labeling with biotin-maleimide reagents, which irreversibly bind to the cysteine thiol that was S-acylated. Biotinylation of the newly formed thiol groups can then be detected on the antibody array. If desired, avidin resin can be used to selectively enrich S-acylated proteins/peptides labeled with biotin. The biotin labeled sample is added into antibody array glass slide wells. The antibody array slide wells are washed. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components