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規(guī)格

規(guī)格	8樣本(產(chǎn)品加服務(wù))、4樣本、8樣本、4樣本(產(chǎn)品加服務(wù))
樣本類(lèi)型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測(cè)方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測(cè)靶標(biāo)	CXCL16, GRO alpha/beta/gamma, Eotaxin-1 / CCL11, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, BLC / CXCL13, MIG / CXCL9, MIP-1 beta / CCL4, CK beta 8-1 / CCL23, MCP-2 / CCL8, MCP-3 / MARC / CCL7, IP-10 / CXCL10, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, MIP-3 alpha / CCL20, MEC / CCL28, CTACK / CCL27, ENA-78 / CXCL5, Eotaxin-2 / MPIF-2 / CCL24, Eotaxin-3 / CCL26, Fractalkine / CX3CL1, GCP-2 / CXCL6, HCC-4 / CCL16, I-309 / TCA-3 / CCL1, I-TAC / CXCL11, Lymphotactin / XCL1, MCP-4 / CCL13, MDC / CCL22, MIP-1 delta / CCL15, MIP-3 beta / CCL19, MPIF-1 / CCL23, NAP-2 / PPBP / CXCL7, PARC / CCL18, SDF-1 alpha / CXCL12 alpha, SDF-1 beta / CXCL12 beta, TARC / CCL17, TECK / CCL25, TCK-1 / CXCL7
靶標(biāo)數(shù)	38

詳細(xì)描述

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	Ck beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA
ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA
GCP-2 (CXCL6) Gene Symbol: CXCL6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876	GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA
I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin? ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA
MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA
MPIF-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human MPIF-1 ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: Ppbp Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA
SDF-1 beta (CXCL12 beta) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 beta ELISA	TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Chemokine Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAH-CHE-G1.pdf

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