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規(guī)格

規(guī)格	4樣本、8樣本、2樣本、4樣本(產(chǎn)品加服務(wù))、2樣本(產(chǎn)品加服務(wù))、8樣本(產(chǎn)品加服務(wù))
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Mouse
檢測靶標	CXCL16, MIP-2, LIX, MCP-5, MIP-1 gamma, Eotaxin-1 / CCL11, MIP-1 alpha / CCL3, RANTES / CCL5, BLC / CXCL13, MIG / CXCL9, CXCL2 / CINC-3 / MIP2, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, MIP-3 alpha / CCL20, 6Ckine / CCL21, CTACK / CCL27, ENA-78 / CXCL5, Eotaxin-2 / MPIF-2 / CCL24, Fractalkine / CX3CL1, I-309 / TCA-3 / CCL1, I-TAC / CXCL11, MDC / CCL22, MIP-3 beta / CCL19, Platelet Factor 4 / CXCL4, TARC / CCL17, TECK / CCL25, SDF-1 / CXCL12
靶標數(shù)	25

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CTACK (CCL27) Gene Symbol: CCL27 Accession #: Q9Z1X0 ELISA Kit: RayBio Mouse CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA
Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA
MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA
RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SDF-1 (CXCL12) Gene Symbol: CXCL12 Accession #: P40224	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Chemokine Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAM-CHE-1.pdf

文獻(4)

Abstract 586: Development of a novel pan-chemokine receptor inhibitor as therapeutic for breast cancer,Hongmei Song, Gang Wang, Bin He, Li Li, Caixia Li, Yusi Lai, Xianghui Xu, Zhongwei Gu,International Journal of Nanomedicine ,(IF:7.033) 作者單位：四川大學(xué)
Species：	Mouse
Sample Type：	serum

View publications

Nociception-Dependent CCL21 Induces Dorsal Root Ganglia Axonal Growth via CCR7-ERK Activation,Yang Xiaosheng, Liu Yang, Zhong Weijie, Li Yi, Zhang Wenchuan,Frontiers in Immunology ,(IF:7.3) 作者單位：上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院
Species：	Mouse
Sample Type：	unknown

View publications

Programming Cell-Derived Vesicles with Enhanced Immunomodulatory Properties,Zhang Rui, Dong Mengxue, Tu Juchuanli, Li Fengkai, Deng Qiaodan, Xu Jiahui, He Xueyan, Ding Jiajun, Xia Jie, Sheng Dandan, Chang Zhaoxia, Ma Wei, Dong Haonan, Zhang Yi, Zhang Lixing, Zhang Lu, Liu Suling,Signal Transduction and Targeted Therapy ,(IF:39.3) 作者單位：復(fù)旦大學(xué)附屬腫癌醫(yī)院
Species：	Mouse
Sample Type：	unknown

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RAI16 maintains intestinal homeostasis and inhibits NLRP3‐dependent IL‐18/CXCL16‐induced colitis and the progression of colitis‐associated colorectal cancer,Deniz Ceylanl?, Ahmet ?zer ?ehirli, Sevgi Gen?osman, Kerem Teral?, Hüseyin ?ah, Nurhayat Gülmez, Serkan Say?ner,Antioxidants ,(IF:7.675)
Species：	Mouse
Sample Type：	colon

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