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規(guī)格

規(guī)格	2樣本、2樣本(產(chǎn)品加服務(wù))、4樣本(產(chǎn)品加服務(wù))、4樣本、8樣本(產(chǎn)品加服務(wù))、8樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Porcine (pig)
檢測靶標(biāo)	IFN-gamma, TNF alpha, IFN alpha, IFN-beta, MIF, CCL3L1, Eotaxin-1 / CCL11, RANTES / CCL5, IL-1 beta / IL-1F2, MIG / CXCL9, MIP-1 beta / CCL4, IL-1 alpha / IL-1F1
靶標(biāo)數(shù)	12

詳細(xì)描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CCL3L1 Accession #: A1YM34 ELISA Kit: RayBio Porcine CCL3L1 ELISA	Eotaxin-1 (CCL11) Accession #: ELISA Kit: RayBio Porcine CCL11 ELISA	IFN alpha Gene ID: Accession #: P49879 ELISA Kit: RayBio Porcine IFN-alpha ELISA	IFN-beta Gene Symbol: interferon-beta-1 IFN-beta IFNB IFNB1 interferon-beta/ poIFN-beta Accession #: Q29412 ELISA Kit: RayBio Porcine IFN-beta ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 396991 Accession #: P17803 ELISA Kit: RayBio Porcine IFN-gamma ELISA
IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 397094 Accession #: P18430 ELISA Kit: RayBio Porcine IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 397122 Accession #: P26889 ELISA Kit: RayBio Porcine IL-1 beta ELISA	MIF Gene Symbol: MIF Gene ID: 397412 Accession #: P80928 ELISA Kit: RayBio Porcine MIF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 100135681 Accession #: B0FYK2 ELISA Kit: RayBio Porcine CXCL9 ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 MIP1B SCY4A Gene ID: 396668 Accession #: Q711P4 ELISA Kit: RayBio Porcine CCL-4/MIP-1 beta ELISA
RANTES (CCL5) Gene Symbol: CCL5 SCYA5 Gene ID: Accession #: Q29288 ELISA Kit: RayBio Porcine RANTES ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 397086 Accession #: P23563 ELISA Kit: RayBio Porcine TNF-alpha ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Porcine Chemokine Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAP-CHE-1.pdf

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