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規(guī)格

規(guī)格	48 tests、192 tests、96 tests、480 tests
樣本類型	Cell Culture Supernatants, Serum
原理方法	Sandwich-based
檢測(cè)方法	Flow Cytometry
定量與否	Quantitative
載體	Fluorescent Microbeads
適用種屬	Human
檢測(cè)靶標(biāo)	IFN-gamma, TNF alpha, IL-10, IL-17A, IL-2, IL-4, IL-6, Fas, Fas Ligand, TNF beta, Granzyme A, TRAIL

詳細(xì)描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 μl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Fas (TNFRSF6/Apo-1) Gene Symbol: FAS APT1 FAS1 TNFRSF6 Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6 Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	Granzyme A Gene Symbol: CTLA3 GZMA HFSP Gene ID: 3001 Accession #: P12544 ELISA Kit: RayBio Human Granzyme A ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA
IL-17A Gene Symbol: IL17A CTLA8 IL17 Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17 ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA
TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRAIL (TNFSF10) Gene Symbol: TNFSF10 APO2L TRAIL Gene ID: 8743 Accession #: P50591 ELISA Kit: RayBio Human TRAIL ELISA	?	?	?

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex? Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio? Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex? bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex? Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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文件

FAH-CTC-1.pdf

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