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規(guī)格

規(guī)格	192 tests、96 tests、480 tests、48 tests
樣本類(lèi)型	Cell Culture Supernatants, Serum
原理方法	Sandwich-based
檢測(cè)方法	Flow Cytometry
定量與否	Quantitative
載體	Fluorescent Microbeads
適用種屬	Mouse
檢測(cè)靶標(biāo)	TRAIL / TNFSF10, IL-6 / IL6, IL-10 / IL10, TNF alpha / TNFA, IL-2 / IL2, IL-4 / IL4, IFN-gamma / IFNG, Fas / Fas Ligand / TNFRSF6 / Apo-1 / FASLG, Granzyme B / GZMB, TNF beta / TNFSF1 / LT-alpha / TNFB

詳細(xì)描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 μL or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

IL-4 Gene Symbol: Il4 Il-4 Gene ID: 16189 Accession #: P07750 ELISA Kit: Mouse IL-4 ELISA Kit	IL-10 Gene Symbol: Il10 Il-10 Gene ID: 16153 Accession #: P18893 ELISA Kit: Mouse IL-10 ELISA Kit	Fas L Gene Symbol: Faslg Apt1lg1 Cd95l Fasl gld Tnfsf6 Gene ID: 14103 Accession #: P41047 ELISA Kit: Mouse Fas L ELISA Kit	Granzyme B Gene Symbol: Gzmb Ctla-1 Ctla1 Gene ID: 14939 Accession #: P04187 ELISA Kit: Mouse Granzyme B ELISA Kit	IL-2 Gene Symbol: Il2 Il-2 Gene ID: 16183 Accession #: P04351 ELISA Kit: Mouse IL-2 ELISA Kit
IL-6 Gene Symbol: Il6 Il-6 Gene ID: 16193 Accession #: P08505 ELISA Kit: Mouse IL-6 ELISA Kit	Fas Gene Symbol: Fas Apt1 Tnfrsf6 Gene ID: 14102 Accession #: P25446 ELISA Kit: Mouse FAS/TNFRSF6/Apo-1 ELISA Kit	TRAIL Gene Symbol: Tnfsf10 Trail Gene ID: 22035 Accession #: P50592 ELISA Kit: Mouse TRAIL ELISA Kit	TNFβ Gene Symbol: Lta Tnfb Tnfsf1 Gene ID: 16992 Accession #: P09225 ELISA Kit: Mouse TNF-beta/LTA/TNFSF1B ELISA Kit	TNFα Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 21926 Accession #: P06804 ELISA Kit: Mouse TNF-alpha ELISA Kit
IFNγ Gene Symbol: Ifng Gene ID: 15978 Accession #: P01580 ELISA Kit: Mouse IFN-gamma ELISA Kit	IL-17A Gene Symbol: Il17a Ctla8 Il17 Gene ID: 16171 Accession #: Q62386 ELISA Kit: Mouse IL-17A ELISA Kit	?	?	?

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex? Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^? Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex? bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex? Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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文件

FAM-CTC-1.pdf

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