Product Features
- Less sample, more data: up to 25 proteins can be quantified from only 25 μl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
IFN-gamma, IL-10, IL-13, IL-17A, IL-2, IL-22, IL-23 p19, IL-4, IL-5, IL-6, IL-9, TNF alpha
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex? Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- Lyophilized Protein Standard Mix
- Biotinylated Detection Antibody Cocktail
- 10X Streptavidin-PE
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
- RayBio? Serum Diluent
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex? bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex? Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
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