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G-Series Human Chemokine Array 1 Kit. Semi-quantitative measurement of 40 human cytokines. Suitable for all liquid sample types.

    請選擇規(guī)格

  • 規(guī)格:
    16樣本
    32樣本
    64樣本
    64樣本(產(chǎn)品加服務(wù))
    32樣本(產(chǎn)品加服務(wù))
    16樣本(產(chǎn)品加服務(wù))
  • 數(shù)量:
  • Human Chemokine Array GS1(GSH-CHE-1)
    14152.32
總計:14152.32

45287.37

以上報價是含稅價格


規(guī)格

貨號:GSH-CHE-1-1
貨號:GSH-CHE-1-1
貨號:GSH-CHE-1-2
貨號:GSH-CHE-1-2
貨號:GSH-CHE-1-4
貨號:GSH-CHE-1-4

貨號:

貨期:2-3周

規(guī)格

規(guī)格 32樣本(產(chǎn)品加服務(wù))、64樣本(產(chǎn)品加服務(wù))、16樣本(產(chǎn)品加服務(wù))、16樣本、32樣本、64樣本
樣本類型 Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法 Sandwich-based
檢測方法 Fluorescence Laser Scanner
定量與否 Semi-Quantitative
載體 Glass Slide
適用種屬 Human
檢測靶標(biāo) LIF, MIF, MSP alpha/beta, CXCL16, OPN, GRO alpha/beta/gamma, AXL, IL-17F, IL-31, IL-9, TSLP, CK beta 8-1 / CCL23, IL-18BPa, LIGHT / TNFSF14, MCP-2 / CCL8, MCP-3 / MARC / CCL7, IP-10 / CXCL10, MIP-3 alpha / CCL20, 6Ckine / CCL21, Betacellulin / BTC, MEC / CCL28, CTACK / CCL27, ENA-78 / CXCL5, Eotaxin-3 / CCL26, GCP-2 / CXCL6, HCC-1 / CCL14, HCC-4 / CCL16, IL-28A / IFN lambda 2, IL-29 / IFN-lambda 1, I-TAC / CXCL11, Lymphotactin / XCL1, MCP-4 / CCL13, MDC / CCL22, MIP-3 beta / CCL19, MPIF-1 / CCL23, NAP-2 / PPBP / CXCL7, Osteopontin / SPP1, PARC / CCL18, Platelet Factor 4 / CXCL4, SDF-1 alpha / CXCL12 alpha, TARC / CCL17, TECK / CCL25, TCK-1 / CXCL7
靶標(biāo)數(shù) 40

詳細描述

Introduction

RayBio? G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody? Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 μl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 μL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
Axl
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
CXCL16
ENA-78
(CXCL5)
Eotaxin-3
(CCL26)
GCP-2
(CXCL6)
GRO
  • Gene Symbol: CXCL1/CXCL2/CXCL3
HCC-1
(CCL14)
HCC-4
(CCL16)
IL-9
IL-17F
IL-18 BP alpha
IL-28A
IL-29
IL-31
IP-10
(CXCL10)
I-TAC
(CXCL11)
LIF
Light
(TNFSF14)
Lymphotactin
(XCL1)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MDC
(CCL22)
MIF
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
Osteopontin
(SPP1)
PARC
(CCL18)
Platelet Factor 4
(CXCL4)
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TECK
(CCL25)
TSLP

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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