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規(guī)格

規(guī)格	50樣本、22樣本、8樣本、8樣本(產(chǎn)品加服務(wù))、50樣本(產(chǎn)品加服務(wù))、22樣本(產(chǎn)品加服務(wù))
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Mouse
檢測靶標	CXCL16, MIP-2, LIX, MCP-5, MIP-1 gamma, Eotaxin-1 / CCL11, MIP-1 alpha / CCL3, RANTES / CCL5, BLC / CXCL13, MIG / CXCL9, CXCL2 / CINC-3 / MIP2, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, MIP-3 alpha / CCL20, 6Ckine / CCL21, CTACK / CCL27, ENA-78 / CXCL5, Eotaxin-2 / MPIF-2 / CCL24, Fractalkine / CX3CL1, I-309 / TCA-3 / CCL1, I-TAC / CXCL11, MDC / CCL22, MIP-3 beta / CCL19, Platelet Factor 4 / CXCL4, SDF-1 alpha / CXCL12 alpha, TARC / CCL17, TECK / CCL25
靶標數(shù)	25

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CTACK (CCL27) Gene Symbol: CCL27 Accession #: Q9Z1X0 ELISA Kit: RayBio Mouse CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA
Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA
LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA
Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Mouse Chemokine Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAM-CHE-1.pdf

文獻(1)

Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy,Ni Jiali, Chen Chen, Wang Shuangan, Liu Xuan, Tan Liping, Lu Li, Fan Yu, Hou Yayi, Dou Huan, Liang Jun,ARTHRITIS RESEARCH & THERAPY ,(IF:4.9) 作者單位：南京鼓樓醫(yī)院
Species：	Mouse
Sample Type：	CT26 cell-Xenograft,serum

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